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Invitrogen AM1908 MEGAclear? Transcription Clean-Up Kit

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  • 产品名称:Invitrogen AM1908 MEGAclear? Transcription Clean-Up Kit
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简单先容

Invitrogen AM1908 MEGAclear? Transcription Clean-Up Kit

产品描述

The MEGAclear™ Kit is designed for the quick cleanup of any large-scale transcription reaction. The MEGAclear™ Kit efficiently separates RNA from unincorporated NTPs, enzymes, and buffer components. Advantages of the MEGAclear™ Kit:

• Purifies transcription reactions to produce high-quality RNA, free of unincorporated NTPs, enzymes, and buffer components

• High recovery from 1 ng to 500 µg of input

• Ideal for purification of aRNA amplification reactions, nonisotopically labeled RNA probes, and capped RNA transcripts for oocyte injections

• Quick protocol—purify RNA in less than 30 minutes

Using the MEGAclear™ Kit

The protocol is quick and easy. The transcription reaction is mixed with Binding Solution, passed over an RNA-binding filter, and washed; an elution step releases the purified RNA, which is now ready for any application requiring highly pure synthetic or in vitrotranscribed RNA. For RNA recovery of 70% or greater, single-stranded RNA should be >100 nt and double-stranded RNA should be >200 bp.

规格

Sample Type (General): RNA

High Throughput Compatibility:   Not High Throughput-Compatible (Manual)

Downstream Application:     Nuclease Protection Assays, Northern Blotting

Format:   Tube(s)

Platform:       Vacuum Manifold, Centrifuge

Product Size: 20 preps

Final Product:       In Vitro Transcribed RNA (Purified)

Yield (up to):  500 µg

Purification Time:  30 min

Number of Reactions:   20 Preps

Isolation Technology:    Silica Spin Column

Sample Type (Specific):       In Vitro Transcribed RNA, Total RNA

Starting Material (Amount):   1 ng to 500 µg of dsRNA>200b or ssRA>100bp

Shipping Condition:      Room Temperature

内容及储存

Binding Solution, Wash Solution Concentrate, and 5 M Ammonium Acetate are stored at 4°C. Elution Solution can be stored at any temperature, and Filter Cartridges and Collection and Elution Tubes are stored at room temperature.

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