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Invitrogen 12800-082 DMEM, powder, high glucose, pyruvate

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  • 产品名称:Invitrogen 12800-082 DMEM, powder, high glucose, pyruvate
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Invitrogen 12800-082 DMEM, powder, high glucose, pyruvate

产品描述

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:

With                 Without

• High Glucose     •  HEPES

• L-glutamine •  Sodium Bicarbonate

• Phenol Red

• Sodium Pyruvate     

The complete formulation is available.

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.

Product Intended Use

For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

规格

Glutamine:                       L-Glutamine

Phenol Red Indicator:              Phenol Red

Form:                               Powder

Glucose:                         High Glucose

Serum Supplementation:        Standard Serum Supplementation

HEPES Buffer:                           No HEPES

Sodium Pyruvate Additive:        Sodium Pyruvate

Product Size:                          10 L

Sodium Bicarbonate Buffer:    No Sodium Bicarbonate

Shipping Condition:                   Room Temperature

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